The series was originally titled Body of Evidence before being renamed Body of Proof.

Although set in Philadelphia, the first season was actually filmed in Providence, Rhode Island, along with other locations like Warwick and Woonsocket.

The medical examiner's office set was actually the GTECH HQ / Providence Warwick Visitors Bureau and surrounding buildings, digitally superimposed on the real Philadelphia skyline.

The second season moved filming to Los Angeles in order to take advantage of a $7 million tax credit.

Real-life technical advisers were on set during filming to ensure medical and forensic procedures were scientifically accurate; actress Jeri Ryan noted their presence helped keep the science realistic.

Body of Proof marked the third ABC series in which Dana Delany played a leading role, following her earlier roles as Colleen McMurphy in China Beach and Katherine Mayfair in Desperate Housewives.

The Region 2 and 4 DVD releases of season 1 present the episodes in production order rather than broadcast order, meaning they include four episodes that were actually filmed during season 1 but aired as part of season 2.

Despite improved ratings, ABC cancelled Body of Proof after three seasons in May 2013; cable networks TNT, USA Network, and WGN America were initially reported as interested in picking it up, but ABC Studios confirmed the series would not move to a new network.