The series finale introduced the 'Spider-Verse' concept involving parallel universe Spider-Men, which later inspired similar multiverse storylines in Spider-Man comics, cartoons, and films, most notably Sony's Spider-Verse animated films.

Writer John Semper Jr. later claimed that the series' 'Spider Wars' arc, adapting the second Clone Saga, directly inspired Dan Slott's comic book 'Spider-Verse' storyline.

Showrunner John Semper watched previous Spider-Man adaptations for inspiration but was unimpressed by most of them, except the live-action 1978 Japanese Spider-Man series, which he found 'great' and 'goofy fun'; its giant mecha robot influenced the final parallel-universe story arc.

Production rules for the show banned Spider-Man from punching enemies, throwing people through glass, putting children in jeopardy, depicting vampires, or using the word 'sinister'; Semper stated the decision for Spider-Man not to use his fists was his own choice to avoid teaching kids that violence solves problems, not a censorship mandate.

Background illustrators researched real aerial photos and maps of New York City to accurately depict rooftops and buildings, but an entire set of animation cels depicting the Pan Am Building had to be scrapped after the California-based art staff discovered the landmark had been renamed and re-signed over a year earlier.

Marvel Films originally wanted fully CGI backgrounds for Spider-Man's web-swinging sequences, but budget constraints forced the production to rely on traditional cel animation, occasionally supplemented by CGI backgrounds created by video game developer Kronos Digital Entertainment.

The show's theme song, performed by Aerosmith guitarist Joe Perry, came about after producer Avi Arad met Perry on a plane flight from New York to Los Angeles and Perry expressed interest in scoring a Spider-Man theme.

Producers were encouraged to use an aggressive rock theme song partly because Saban had already found success with a similarly hard-edged opening theme for Mighty Morphin Power Rangers in 1993, which shifted the company's previous hesitance about using such music for children's programming.

Aerosmith was referenced within the show itself: in the 1995 episode 'The Alien Costume, Part One,' Peter Parker briefly transforms into a member of the band.

Composer Haim Saban was credited in the closing credits under the pseudonym 'Kussa Mahchi,' an alias he also used on other shows he produced music for, including Mighty Morphin Power Rangers, X-Men, and the English dub of Dragon Ball Z.